3-D organoids and RNA sequencing reveal the crosstalk driving lung cell formation


(Boston Children’s Hospital) To investigate lung-cell formation, researchers used a 3-D ‘organoid’ culture system allowing them to observe what kinds of cells were being made. To this, they added single-cell RNA sequencing to see what genes each cell type turns on, which enabled them to identify what the cells are saying to each other. As reported today in Cell, they found that lung cells known as mesenchymal cells are the cells driving the action.